Gene description for TSG101
Gene name tumor susceptibility 101
Gene symbol TSG101
Other names/aliases TSG10
VPS23
Species Homo sapiens
 Experiment description of studies that identified TSG101 in Vascular smooth muscle cells

1
Experiment ID2012
Identified moleculeprotein
Extracellular vesicle typeLarge extracellular vesicles
MISEVLarge EVs
Identification method Western blotting
PubMed ID 36433666   
OrganismHomo sapiens
Experiment descriptionVascular smooth muscle cell senescence accelerates medin aggregation via small extracellular vesicle secretion and extracellular matrix reorganization
AuthorsWhitehead M, Yusoff S, Ahmad S, Schmidt L, Mayr M, Madine J, Middleton D, Shanahan CM.
Journal name Aging Cell
Publication year2023
SampleVascular smooth muscle cells
Sample nameAorta-derived VSMCs
Isolation/purification methodsCentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

2
Experiment ID2013
Identified moleculeprotein
Extracellular vesicle typeMedium extracellular vesicles
MISEVLarge EVs
Identification method Western blotting
PubMed ID 36433666   
OrganismHomo sapiens
Experiment descriptionVascular smooth muscle cell senescence accelerates medin aggregation via small extracellular vesicle secretion and extracellular matrix reorganization
AuthorsWhitehead M, Yusoff S, Ahmad S, Schmidt L, Mayr M, Madine J, Middleton D, Shanahan CM.
Journal name Aging Cell
Publication year2023
SampleVascular smooth muscle cells
Sample nameAorta-derived VSMCs
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

3
Experiment ID2014
Identified moleculeprotein
Extracellular vesicle typeSmall extracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36433666   
OrganismHomo sapiens
Experiment descriptionVascular smooth muscle cell senescence accelerates medin aggregation via small extracellular vesicle secretion and extracellular matrix reorganization
AuthorsWhitehead M, Yusoff S, Ahmad S, Schmidt L, Mayr M, Madine J, Middleton D, Shanahan CM.
Journal name Aging Cell
Publication year2023
SampleVascular smooth muscle cells
Sample nameAorta-derived VSMCs
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

4
Experiment ID2015
Identified moleculeprotein
Extracellular vesicle typeLarge extracellular vesicles
MISEVLarge EVs
Identification method Western blotting
PubMed ID 36433666   
OrganismHomo sapiens
Experiment descriptionVascular smooth muscle cell senescence accelerates medin aggregation via small extracellular vesicle secretion and extracellular matrix reorganization
AuthorsWhitehead M, Yusoff S, Ahmad S, Schmidt L, Mayr M, Madine J, Middleton D, Shanahan CM.
Journal name Aging Cell
Publication year2023
SampleVascular smooth muscle cells
Sample nameAorta-derived VSMCs
Isolation/purification methodsCentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

5
Experiment ID2016
Identified moleculeprotein
Extracellular vesicle typeMedium extracellular vesicles
MISEVLarge EVs
Identification method Western blotting
PubMed ID 36433666   
OrganismHomo sapiens
Experiment descriptionVascular smooth muscle cell senescence accelerates medin aggregation via small extracellular vesicle secretion and extracellular matrix reorganization
AuthorsWhitehead M, Yusoff S, Ahmad S, Schmidt L, Mayr M, Madine J, Middleton D, Shanahan CM.
Journal name Aging Cell
Publication year2023
SampleVascular smooth muscle cells
Sample nameAorta-derived VSMCs
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

6
Experiment ID2017
Identified moleculeprotein
Extracellular vesicle typeSmall extracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36433666   
OrganismHomo sapiens
Experiment descriptionVascular smooth muscle cell senescence accelerates medin aggregation via small extracellular vesicle secretion and extracellular matrix reorganization
AuthorsWhitehead M, Yusoff S, Ahmad S, Schmidt L, Mayr M, Madine J, Middleton D, Shanahan CM.
Journal name Aging Cell
Publication year2023
SampleVascular smooth muscle cells
Sample nameAorta-derived VSMCs
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

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